Sonoporation Enhances Liposome Mediated EcTRAF4 siRNA Delivery in SaB-1 Cells

Sonoporation, which is the perforation of cell membranes using ultrasound and microbubble, has been shown to facilitate liposome-mediated delivery nucleotides in mammalian cells. The effect not yet tested non-vertebrate species such as fish. Since adenoviral vector associated with tumorigenicity electroporation affected by ions content culture water, sonoporation may be an ideal means enhancing transfection efficiency aquaculture. Hence, this study, was used enhance siEcTRAF4 lipoplexes, inhibits Viral Haemorrhagic Septicaemia Virus (VHSV) infection, SaB-1 However, parameters on must elucidated, so acoustical pressures bubble-to-cell distance membrane permeabilization cells analyzed. Sonoporation found assist lipoplexes into reduce viral replication after VHSV challenge. These results imply that a prognostic technique against infection combines applicable aquaculture future.